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important role in controlling GBS infection when mice are infected with low doses of bacteria [17]. Infections with lower S. suis concentrations (#56106 CFU/mL) did not induce clinical disease in any of the mouse strains tested (results not shown). It is important to note that the pathogenesis of S. suis and GBS differ mainly in that the capsular polysaccharide of these pathogens present high or l
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The buffer was 20 mM imidazole pH seven, with the addition of one mM DTT and 1 mM EDTA when T6P synthase was assayed. The extract was centrifuged in the cold for 15 min at 13000 rpm in an Eppendorf desk leading centrifuge and the supernatant utilized for willpower of enzyme activities. T6P synthase action was decided by a two phase strategy in a combination of 50 mM imidazole pH seven, .one M KCl,
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When Y. lipolytica diploids homozygous for the tps1 mutation (CJM 724) had been positioned in sporulation circumstances the sporulation frequency was decreased with respect to that of wild kind (CJM 722) or heterozygous TPS1/tps1 (CJM 723) diploids. A comparable conduct in tps1/tps1 diploids in S. cerevisiae was ascribed to a decreased expression of MCK1, a gene that stimulates expression of IME1
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